Preparation of32P-labelled adenosine triphosphate

A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Adenosine triphosphate inhibition in myosin adenosine triphosphate systems.

Factors affecting inhibition of myosin ATPase by ATP, such as concentration of the enzyme, of ATP, and of the divalent metals, calcium and magnesium, have been studied and the results are reported here. Experiments were carried out under conditions in which no known magnesium effects occurred, so that only the effect of calcium on the nucleoside triphosphatase activity of myosin A could be qual...

The Preparation and Properties of IV-Hydroxyethyl Derivatives of Adenosine, Adenosine Triphosphate, and Nicotinamide Adenine Dinucleotide*

Previous work shows that replacement of the nicotinamide moiety of nicotinamide adenine dinucleotide with other substituted pyridines leads to a useful series of analogues of this coenzyme (3, 4). The present communication describes the preparation and properties of a different series of analogues obtained by systematic modification of the adenine moiety of NAD. The only adenine-modified analog...

The preparation and properties of N-hydroxyethyl derivatives of adenosine, adenosine triphosphate, and nicotinamide adenine dinucleotide.

Previous work shows that replacement of the nicotinamide moiety of nicotinamide adenine dinucleotide with other substituted pyridines leads to a useful series of analogues of this coenzyme (3, 4). The present communication describes the preparation and properties of a different series of analogues obtained by systematic modification of the adenine moiety of NAD. The only adenine-modified analog...

Adenosine Triphosphate-dependent Taurocholate

Transport systems involved in uptake and biliary secretion of bile salts have been extensively studied in rat liver; however, little is known about these systems in the human liver. In this study, we investigated taurocholate (TC) transport in canalicular and basolateral plasma membrane vesicles isolated from 15 human livers (donor age 6-64 yr). ATP stimulated the uptake ofTC into both canalicu...